Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry

Int J Mol Sci. 2015 Dec 2;16(12):28566-81. doi: 10.3390/ijms161226120.


The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach.

Keywords: PRM; Q-Exactive; SRM; accurate mass; high resolution; mass spectrometry; parallel reaction monitoring; proteomics; quantitative proteomics; selected reaction monitoring; targeted.

Publication types

  • Review

MeSH terms

  • Animals
  • Humans
  • Mass Spectrometry / methods*
  • Mass Spectrometry / standards
  • Peptides / chemistry
  • Peptides / metabolism
  • Proteins / chemistry
  • Proteins / metabolism
  • Proteomics / methods*
  • Proteomics / standards


  • Peptides
  • Proteins