Wasabi: An Integrated Platform for Evolutionary Sequence Analysis and Data Visualization

Mol Biol Evol. 2016 Apr;33(4):1126-30. doi: 10.1093/molbev/msv333. Epub 2015 Dec 3.


Wasabi is an open source, web-based environment for evolutionary sequence analysis. Wasabi visualizes sequence data together with a phylogenetic tree within a modern, user-friendly interface: The interface hides extraneous options, supports context sensitive menus, drag-and-drop editing, and displays additional information, such as ancestral sequences, associated with specific tree nodes. The Wasabi environment supports reproducibility by automatically storing intermediate analysis steps and includes built-in functions to share data between users and publish analysis results. For computational analysis, Wasabi supports PRANK and PAGAN for phylogeny-aware alignment and alignment extension, and it can be easily extended with other tools. Along with drag-and-drop import of local files, Wasabi can access remote data through URL and import sequence data, GeneTrees and EPO alignments directly from Ensembl. To demonstrate a typical workflow using Wasabi, we reproduce key findings from recent comparative genomics studies, including a reanalysis of the EGLN1 gene from the tiger genome study: These case studies can be browsed within Wasabi at http://wasabiapp.org:8000?id=usecases. Wasabi runs inside a web browser and does not require any installation. One can start using it at http://wasabiapp.org. All source code is licensed under the AGPLv3.

Keywords: data visualization; evolutionary sequence analysis; reproducible research.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Evolution, Molecular*
  • Genome
  • Internet
  • Phylogeny*
  • Sequence Alignment
  • Sequence Analysis / methods*
  • Sequence Analysis, DNA
  • Software*
  • Web Browser