Alterations of telomere length and DNA methylation in hairdressers: A cross-sectional study

Environ Mol Mutagen. 2016 Mar;57(2):159-67. doi: 10.1002/em.21991. Epub 2015 Dec 6.

Abstract

Working as hairdressers has been associated with increased risk for cancer, particularly bladder cancer. To evaluate if current hairdressers have elevated risks of adverse health effects, we measured several biomarkers related to cancer-related DNA alterations. We enrolled 295 hairdressers and 92 non-hairdressers (all female non-smokers) from Stockholm and southern Sweden. Questionnaire data were collected for each participant, including work tasks for the hairdressers. We measured telomere length in peripheral blood leucocytes using quantitative PCR and DNA methylation status of genes relevant for bladder cancer using methylation sensitive high resolution melting analysis. The hairdressers had shorter telomeres (β = -0.069, P = 0.019) compared with non-hairdressers. Shorter telomeres were found in hairdressers up to 32 years old performing hair waving more than once per week as compared with hairdressers in the same age group performing hair waving less often (β = -0.12, P = 0.037). Hair waving was associated with less frequent CDKN2A methylation (odds ratio, OR = 0.19, P = 0.033). Shorter telomeres in hairdressers may indicate a genotoxic effect. Performing hair waving was associated with short telomere length, although the effect was only observed in young hairdressers. No clear patterns were discerned with regard to DNA methylation of bladder cancer-related genes. The observed changes of methylation were not all in the expected direction and warrant further investigation.

Keywords: cancer risk; epigenetic; genotoxicity; occupational exposure; toluidine.

MeSH terms

  • Adult
  • Aging / genetics
  • Case-Control Studies
  • Cross-Sectional Studies
  • Cyclin-Dependent Kinase Inhibitor p16 / genetics
  • DNA Methylation*
  • DNA Modification Methylases / genetics
  • DNA Repair Enzymes / genetics
  • Female
  • Glutathione S-Transferase pi / genetics
  • Humans
  • Middle Aged
  • Nuclear Proteins / genetics
  • Occupational Diseases / etiology
  • Occupational Exposure / adverse effects*
  • Sweden
  • Telomere*
  • Time Factors
  • Tumor Suppressor Proteins / genetics
  • Twist-Related Protein 1 / genetics
  • Young Adult

Substances

  • Cyclin-Dependent Kinase Inhibitor p16
  • Nuclear Proteins
  • TWIST1 protein, human
  • Tumor Suppressor Proteins
  • Twist-Related Protein 1
  • DNA Modification Methylases
  • MGMT protein, human
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • DNA Repair Enzymes