Layer by layer assembly of albumin nanoparticles with selective recognition of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)

J Colloid Interface Sci. 2016 Mar 1:465:11-7. doi: 10.1016/j.jcis.2015.11.054. Epub 2015 Nov 28.

Abstract

Crosslinked albumin nanoparticles which loaded with doxorubicin (DOX) were fabricated with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and biocompatible polysaccharide, alginate (ALG), using layer-by-layer technique. Albumin nanoparticles exhibited narrow size distribution and fluorescent property. The assembled core/shell structure of the nanoparticles can be internalized more easily with the cancer cells, which attributes to TRAIL binding with death receptors. TRAIL still hold bioactive properties after assembled onto the particles. In addition, after loaded into the albumin core nanoparticles, DOX (as the chemotherapeutics) display a synergistic cytotoxic effect on cytotoxicity in combination with TRAIL in vitro. The core/shell nanostructured nanoparticles realized in this study would be used as a promising candidate for novel drug carriers.

Keywords: Albumin; Core–shell; Layer-by-layer; Nanoparticles; TRAIL.

MeSH terms

  • Albumins* / chemistry
  • Albumins* / pharmacology
  • Doxorubicin* / chemistry
  • Doxorubicin* / pharmacology
  • Drug Carriers* / chemistry
  • Drug Carriers* / pharmacology
  • Female
  • Humans
  • MCF-7 Cells
  • Nanoparticles / chemistry*
  • TNF-Related Apoptosis-Inducing Ligand* / chemistry
  • TNF-Related Apoptosis-Inducing Ligand* / pharmacology

Substances

  • Albumins
  • Drug Carriers
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Doxorubicin