Enzymatic in situ saccharification of chestnut shell with high ionic liquid-tolerant cellulases from Galactomyces sp. CCZU11-1 in a biocompatible ionic liquid-cellulase media

Bioresour Technol. 2016 Feb:201:133-9. doi: 10.1016/j.biortech.2015.11.034. Epub 2015 Nov 28.

Abstract

In this study, it was the first time to report that the cellulases of Galactomyces sp. CCZU11-1 showed high activity and stability in the culture and reaction media containing IL [Mmim]DMP. Using untreated chestnut shell (CNS) as carbon source in the culture media containing IL [Mmim]DMP (5%, w/v), high activity of FPA (28.6U/mL), xylanase (186.2U/mL), and CMCase (107.3U/mL) were obtained, and 184.9mg/L of total protein was achieved. Furthermore, the changes in the structural features (crystallinity, morphology, and porosity) of the solid residue of CNS utilized with Galactomyces sp. CCZU11-1 were characterized with Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. After was enzymatically hydrolyzed with the prepared crude enzymes in IL diluted to 20% (w/v), a high yield of reducing sugars, 62.1%, was obtained. Significantly, Galactomyces sp. CCZU11-1 showed high potential for the efficient transformation of lignocellulosic materials to glucose in a single-step process.

Keywords: Biocompatibility; Cellulase; Galactomyces sp. CCZU11-1; In situ saccharification; Ionic liquid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellulase / chemistry*
  • Cellulases / chemistry*
  • Cellulose / chemistry*
  • Culture Media
  • Eleocharis / chemistry*
  • Enzyme Assays
  • Hydrolysis
  • Ionic Liquids / chemistry
  • Saccharomycetales / enzymology*
  • X-Ray Diffraction

Substances

  • Culture Media
  • Ionic Liquids
  • Cellulose
  • Cellulases
  • Cellulase
  • carboxymethylcellulase