Advancing the use of Lactobacillus acidophilus surface layer protein A for the treatment of intestinal disorders in humans

Gut Microbes. 2015;6(6):392-7. doi: 10.1080/19490976.2015.1107697.

Abstract

Intestinal immunity is subject to complex and fine-tuned regulation dictated by interactions of the resident microbial community and their gene products with host innate cells. Deterioration of this delicate process may result in devastating autoinflammatory diseases, including inflammatory bowel disease (IBD), which primarily comprises Crohn's disease (CD) and ulcerative colitis (UC). Efficacious interventions to regulate proinflammatory signals, which play critical roles in IBD, require further scientific investigation. We recently demonstrated that rebalancing intestinal immunity via the surface layer protein A (SlpA) from Lactobacillus acidophilus NCFM potentially represents a feasible therapeutic approach to restore intestinal homeostasis. To expand on these findings, we established a new method of purifying bacterial SlpA, a new SlpA-specific monoclonal antibody, and found no SlpA-associated toxicity in mice. Thus, these data may assist in our efforts to determine the immune regulatory efficacy of SlpA in humans.

Keywords: bacterial protein isolation; colonic inflammation; gut microbiota; intestinal immune regulation; surface layer protein A.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal / immunology
  • Bacterial Proteins / immunology
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / therapeutic use*
  • Bacterial Proteins / toxicity
  • Biological Therapy*
  • Gastrointestinal Microbiome
  • Homeostasis
  • Intestinal Diseases / therapy*
  • Intestines / microbiology
  • Lactobacillus acidophilus* / chemistry
  • Lactobacillus acidophilus* / immunology
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Bacterial Proteins
  • surface layer protein A, Bacteria