Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds

Charlotte Balière; Alain Rincé; Jorge Blanco; Ghizlane Dahbi; Josée Harel; Philippe Vogeleer; Jean-Christophe Giard; Patricia Mariani-Kurkdjian; Michèle Gourmelon

doi:10.3389/fmicb.2015.01356

Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds

Front Microbiol. 2015 Dec 1:6:1356. doi: 10.3389/fmicb.2015.01356. eCollection 2015.

Authors

Charlotte Balière¹, Alain Rincé², Jorge Blanco³, Ghizlane Dahbi³, Josée Harel⁴, Philippe Vogeleer⁴, Jean-Christophe Giard⁵, Patricia Mariani-Kurkdjian⁶, Michèle Gourmelon¹

Affiliations

¹ Laboratoire Santé Environnement et Microbiologie, Unité Santé, Génétique et Microbiologie des Mollusques, Département Ressources Biologiques et Environnement, Ifremer Plouzané, France.
² U2RM EA4655 Stress/Virulence, Normandie-Université, University of Caen Normandy Caen, France.
³ Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela Lugo, Spain.
⁴ Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Centre de Recherche d'Infectiologie Porcine et Avicole, Université de Montréal Saint-Hyacinthe, QC, Canada.
⁵ U2RM EA4655 Antibio-Résistance, Normandie-Université, University of Caen Normandy Caen, France.
⁶ Service de Microbiologie, CNR Associé Escherichia coli, AP-HP, Hôpital Robert-Debré Paris, France ; Infection, Antimicrobials, Modelling, Evolution, UMR 1137, INSERM Paris, France ; Infection, Antimicrobials, Modelling, Evolution, UMR 1137, Université Paris Diderot - Sorbonne Paris Cité Paris, France.

Abstract

more strains formed a strong biofilm at 18 than at 30°C. Finally, more than 85% of analyzed strains were found to be sensitive to the 16 tested antibiotics. These data suggest the low risk of human infection by STEC if shellfish from these shellfish-harvesting areas were consumed.

Keywords: EPEC; MLST; PFGE; STEC; biofilms; shellfish; water.