Effects of Lactobacillus plantarum NCU116 on Intestine Mucosal Immunity in Immunosuppressed Mice

J Agric Food Chem. 2015 Dec 30;63(51):10914-20. doi: 10.1021/acs.jafc.5b04757. Epub 2015 Dec 18.

Abstract

The effects of Lactobacillus plantarum (L. plantarum) NCU116 isolated from pickled vegetables on intestine mucosal immunity in cyclophosphamide treated mice were investigated. Animals were divided into six groups: normal group (NIM), immunosuppression group (IM), immunosuppression plus L. plantarum NCU116 groups with three different doses (NCU-H, NCU-M, and NCU-L), and plus Bifidobacterium BB12 as positive control group (BB12). Results showed that the thymus indexes of the four treatment groups were significantly higher than that of the IM group (2.02 ± 0.16) (p < 0.05) and close to the index of the NIM group (2.61 ± 0.37) at 10 days. The level of immune factor IL-2 notably increased (IM, 121 ± 9.0) (p < 0.05) and was close to 65% of NIM group's level (230 ± 10.7). The levels of other immune factors (IFN-γ, IL-10, IL-12p70, and sIgA), the gene expression levels of IL-2 and IFN-γ, and the number of IgA-secreting cells showed similar patterns (p < 0.05). However, the level of immune factor IL-4 remarkably decreased (IM, 128 ± 10.2) (p < 0.05) and was only approximately 50% of the NIM group (154 ± 18.2). The levels of other immune factors (IL-6 and IgE) and the gene expression level of IL-6 at 10 days exhibited similar changes (p < 0.05) but showed a slight recovery at 20 days, accompanied by the altered protein expression levels of T-bet and GATA-3 in the small intestine. These findings suggest that L. plantarum NCU116 enhanced the immunity of the small intestine in the immunosuppressed mice.

Keywords: Lactobacillus plantarum NCU116; Th1/Th2 balance; cyclophosphamide; immunomodulation; intestinal mucosal immune.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Count
  • Cyclophosphamide / administration & dosage
  • Cytokines / analysis
  • Cytokines / genetics
  • Female
  • GATA3 Transcription Factor / analysis
  • Immunoglobulin A / metabolism
  • Immunosuppression*
  • Intestinal Mucosa / immunology*
  • Intestine, Small / chemistry
  • Intestine, Small / cytology
  • Intestine, Small / immunology
  • Lactobacillus plantarum / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Probiotics
  • RNA, Messenger / analysis
  • Specific Pathogen-Free Organisms
  • T-Box Domain Proteins / analysis

Substances

  • Cytokines
  • GATA3 Transcription Factor
  • Gata3 protein, mouse
  • Immunoglobulin A
  • RNA, Messenger
  • T-Box Domain Proteins
  • T-box transcription factor TBX21
  • Cyclophosphamide