A new role for PGRP-S (Tag7) in immune defense: lymphocyte migration is induced by a chemoattractant complex of Tag7 with Mts1

E A Dukhanina; T I Lukyanova; E A Romanova; V Guerriero; N V Gnuchev; G P Georgiev; D V Yashin; L P Sashchenko

doi:10.1080/15384101.2015.1104440

A new role for PGRP-S (Tag7) in immune defense: lymphocyte migration is induced by a chemoattractant complex of Tag7 with Mts1

Cell Cycle. 2015;14(22):3635-43. doi: 10.1080/15384101.2015.1104440.

Authors

E A Dukhanina^{1

2}, T I Lukyanova^{1

3}, E A Romanova¹, V Guerriero⁴, N V Gnuchev¹, G P Georgiev¹, D V Yashin¹, L P Sashchenko¹

Affiliations

¹ a Institute of Gene Biology; Russian Academy of Sciences (RAS) ; Moscow , Russia.
² b Engelhardt Institute of Molecular Biology; RAS ; Moscow , Russia.
³ c M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry; RAS ; Moscow , Russia.
⁴ d School of Animal and Comparative Biomedical Sciences; University of Arizona ; Tucson , AZ USA.

Abstract

PGRP-S (Tag7) is an innate immunity protein involved in the antimicrobial defense systems, both in insects and in mammals. We have previously shown that Tag7 specifically interacts with several proteins, including Hsp70 and the calcium binding protein S100A4 (Mts1), providing a number of novel cellular functions. Here we show that Tag7-Mts1 complex causes chemotactic migration of lymphocytes, with NK cells being a preferred target. Cells of either innate immunity (neutrophils and monocytes) or acquired immunity (CD4(+) and CD8(+) lymphocytes) can produce this complex, which confirms the close connection between components of the 2 branches of immune response.

Keywords: Mts1; NK cells; Tag7; Tag7-Mts1 complex; chemotaxis; human lymphocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptive Immunity
CD4-Positive T-Lymphocytes / cytology
CD4-Positive T-Lymphocytes / drug effects
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / cytology
CD8-Positive T-Lymphocytes / drug effects
CD8-Positive T-Lymphocytes / immunology
Cell Separation
Chemotaxis, Leukocyte / genetics
Chemotaxis, Leukocyte / immunology*
Cyclin-Dependent Kinase Inhibitor p16 / genetics
Cyclin-Dependent Kinase Inhibitor p16 / immunology*
Cyclin-Dependent Kinase Inhibitor p16 / pharmacology
Cytokines / genetics
Cytokines / immunology*
Cytokines / pharmacology
Escherichia coli
Gene Expression Regulation
Humans
Immunity, Innate*
Killer Cells, Natural / cytology
Killer Cells, Natural / drug effects
Killer Cells, Natural / immunology*
Monocytes / cytology
Monocytes / drug effects
Monocytes / immunology
Neutrophils / cytology
Neutrophils / drug effects
Neutrophils / immunology
Primary Cell Culture
Protein Binding
Recombinant Proteins
Signal Transduction

Substances

Cyclin-Dependent Kinase Inhibitor p16
Cytokines
PGLYRP1 protein, human
Recombinant Proteins