LncRNA-UCA1 exerts oncogenic functions in non-small cell lung cancer by targeting miR-193a-3p

Cancer Lett. 2016 Feb 1;371(1):99-106. doi: 10.1016/j.canlet.2015.11.024. Epub 2015 Dec 3.

Abstract

Recently, the long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) has been identified as an oncogenic gene in multiple human tumor entitles, and dysregulation of UCA1 was tightly linked to carcinogenesis and cancer progression. However, whether the aberrant expression of UCA1 in non-small cell lung cancer (NSCLC) is associated with malignancy, metastasis or prognosis has not been characterized. In this study, we found that UCA1 was upregulated in NSCLC tissues. Higher expression of UCA1 led to a significantly poorer survival time, and multivariate analysis revealed that UCA1 was an independent risk factor of prognosis. UCA1 overexpression enhanced, whereas UCA1 silencing impaired the proliferation and colony formation of NSCLC cells. Moreover, mechanistic investigations showed that UCA1 upregulated the expression of miR-193a-3p target gene ERBB4 through competitively 'spongeing' miR-193a-3p. Overall, we concluded that UCA1 functions as an oncogene in NSCLC, acting mechanistically by upregulating ERBB4 in part through 'spongeing' miR-193a-3p.

Keywords: ERBB4; Non-small cell lung cancer; UCA1; lncRNA; miR-193a-3p.

MeSH terms

  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Carcinoma, Non-Small-Cell Lung / metabolism
  • Carcinoma, Non-Small-Cell Lung / mortality
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Carcinoma, Non-Small-Cell Lung / therapy
  • Cell Line, Tumor
  • Cell Proliferation
  • Chi-Square Distribution
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kaplan-Meier Estimate
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / mortality
  • Lung Neoplasms / pathology
  • Lung Neoplasms / therapy
  • Male
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Middle Aged
  • Multivariate Analysis
  • Oncogenes*
  • Proportional Hazards Models
  • RNA Interference
  • RNA, Long Noncoding / genetics*
  • RNA, Long Noncoding / metabolism
  • Receptor, ErbB-4 / genetics
  • Receptor, ErbB-4 / metabolism
  • Retrospective Studies
  • Risk Factors
  • Time Factors
  • Transfection
  • Treatment Outcome
  • Up-Regulation

Substances

  • MIRN193 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • UCA1 RNA, human
  • ERBB4 protein, human
  • Receptor, ErbB-4