The Therapeutic Potential of Resistant Starch in Modulation of Insulin Resistance, Endotoxemia, Oxidative Stress and Antioxidant Biomarkers in Women with Type 2 Diabetes: A Randomized Controlled Clinical Trial

Ann Nutr Metab. 2016;68(2):85-93. doi: 10.1159/000441683. Epub 2015 Dec 12.


Aims: This trial aims to determine the effects of resistant starch (RS) subtype 2 (RS2) on glycemic status, metabolic endotoxemia and markers of oxidative stress.

Methods: A randomized, controlled, parallel-group clinical trial group of 56 females with type 2 diabetes mellitus (T2DM) was divided to 2 groups. The intervention group (n = 28) and control group (n = 28) received 10 g/day RS2 or placebo for 8 weeks, respectively. Fasting blood samples were taken to determine glycemic status, endotoxin, high sensitivity C-reactive protein (hs-CRP), malondialdehyde (MDA), total antioxidant capacity (TAC), antioxidant enzymes concentrations as well as uric acid at baseline and after the intervention.

Results: After 8 weeks, RS2 caused a significant decrease in the levels of MDA (-34.10%), glycosylated hemoglobin (-9.40%), insulin (-29.36%), homeostasis model of insulin resistance (-32.85%) and endotoxin (-25.00%), a significant increase in TAC (18.10%) and glutathione peroxidase (11.60%) as compared with control. No significant changes were observed in fasting plasma glucose, quantitative insulin sensitivity check index, hs-CRP, superoxide dismutase, catalase and uric acid in the RS2 group as compared with the control group.

Conclusion: Supplementation with RS2 may be improved glycemic status, endotoxemia and markers of oxidative stress in patients with T2DM.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antioxidants / metabolism*
  • Biomarkers
  • Blood Glucose
  • Body Weight
  • C-Reactive Protein / analysis
  • C-Reactive Protein / metabolism
  • Diabetes Mellitus, Type 2 / diet therapy*
  • Endotoxemia / drug therapy*
  • Endotoxins / blood
  • Female
  • Humans
  • Insulin Resistance*
  • Malondialdehyde / metabolism
  • Middle Aged
  • Oxidative Stress / drug effects*
  • Starch / therapeutic use*
  • Uric Acid / metabolism


  • Antioxidants
  • Biomarkers
  • Blood Glucose
  • Endotoxins
  • Uric Acid
  • Malondialdehyde
  • Starch
  • C-Reactive Protein