Proteomic analysis of fatty-acylated proteins

Curr Opin Chem Biol. 2016 Feb:30:77-86. doi: 10.1016/j.cbpa.2015.11.008. Epub 2015 Dec 2.


Protein fatty-acylation in eukaryotes has been associated with many fundamental biological processes. However, the diversity, abundance and regulatory mechanisms of protein fatty-acylation in vivo remain to be explored. Herein, we review the proteomic analysis of fatty-acylated proteins, with a focus on N-myristoylation and S-palmitoylation. We then highlight major challenges and emerging methods for direct site identification, quantitation, and lipid structure characterization to understand the functions and regulatory mechanisms of fatty-acylated proteins in physiology and disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Acylation
  • Animals
  • Fatty Acids / metabolism*
  • Humans
  • Protein Processing, Post-Translational
  • Proteins / metabolism*
  • Proteomics / methods*


  • Fatty Acids
  • Proteins