Identification and Comparative Expression Profiles of Chemoreception Genes Revealed from Major Chemoreception Organs of the Rice Leaf Folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

PLoS One. 2015 Dec 11;10(12):e0144267. doi: 10.1371/journal.pone.0144267. eCollection 2015.

Abstract

To better understand the olfactory mechanisms in the rice leaf folder, Cnaphalocrocis medinalis (Guenée), a serious pest of rice in Asia, we established six partial transcriptomes from antennae, protarsus, and reproductive organs of male and female adults. A total of 102 transcripts were identified, including 29 odorant receptors (ORs), 15 ionotropic receptors (IRs), 30 odorant-binding proteins (OBPs), 26 chemosensory proteins (CSPs), and 2 sensory neuron membrane proteins (SNMPs). The expression patterns of these genes were calculated by fragments per kilobase of exon per million fragments mapped (FPKM) and validated by real-time quantitative PCR (RT-qPCR). Some transcripts were exclusively expressed in specific organs, such as female protarsus, whereas others were universally expressed, this varied expression profile may provide insights into the specific functions mediated by chemoreception proteins in insects. To the best of our knowledge, among the 102 identified transcripts, 81 are novel and have never been reported before. In addition, it also is the first time that ORs and IRs are identified in C. medinalis. Our findings significantly enhance the currently limited understanding olfactory mechanisms of the olfactory mechanisms underlying the chemoreception system in C. medinalis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arthropod Antennae / metabolism
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation
  • Gene Ontology
  • Genes, Insect*
  • Insect Proteins / chemistry
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Molecular Sequence Annotation
  • Molecular Sequence Data
  • Moths / genetics*
  • Organ Specificity / genetics*
  • Oryza / parasitology*
  • Phylogeny
  • Plant Leaves / parasitology*
  • Receptors, Odorant / chemistry
  • Receptors, Odorant / genetics
  • Receptors, Odorant / metabolism
  • Reproducibility of Results
  • Sensory Receptor Cells / metabolism

Substances

  • Insect Proteins
  • Membrane Proteins
  • Receptors, Odorant
  • odorant-binding protein

Grants and funding

This study was supported and funded by the National High Technology Research and Development Program of China (863 Program) (No. 2014AA10A605), the China Agriculture Research System (Grant No. CARS-01-17) and the Fundamental Research Funds for the Central Universities (2013PY046). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.