miR-430 regulates oriented cell division during neural tube development in zebrafish

Dev Biol. 2016 Jan 15;409(2):442-50. doi: 10.1016/j.ydbio.2015.11.016. Epub 2015 Nov 30.


MicroRNAs have emerged as critical regulators of gene expression. Originally shown to regulate developmental timing, microRNAs have since been implicated in a wide range of cellular functions including cell identity, migration and signaling. miRNA-430, the earliest expressed microRNA during zebrafish embryogenesis, is required to undergo morphogenesis and has previously been shown to regulate maternal mRNA clearance, Nodal signaling, and germ cell migration. The functions of miR-430 in brain morphogenesis, however, remain unclear. Herein we find that miR-430 instructs oriented cell divisions in the neural rod required for neural midline formation. Loss of miR-430 function results in mitotic spindle misorientation in the neural rod, failed neuroepithelial integration after cell division, and ectopic cell accumulation in the dorsal neural tube. We propose that miR-430, independently of canonical apicobasal and planar cell polarity (PCP) pathways, coordinates the stereotypical cell divisions that instruct neural tube morphogenesis.

Keywords: MicroRNAs; Neural tube; Zebrafish.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / genetics*
  • Cell Polarity
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / metabolism
  • Gastrulation
  • Gene Expression Regulation, Developmental
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Models, Biological
  • Morphogenesis / genetics
  • Neural Tube / cytology
  • Neural Tube / embryology*
  • Neuroepithelial Cells / cytology
  • Neuroepithelial Cells / metabolism
  • Ribonuclease III / metabolism
  • Spindle Apparatus / metabolism
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Up-Regulation / genetics
  • Zebrafish / embryology*
  • Zebrafish / genetics*


  • MicroRNAs
  • Mirn430 microRNA, zebrafish
  • Ribonuclease III