Performance Characteristics of the New Abbott Real Time MTB Assay for Detection of Mycobacterium Tuberculosis Complex in Respiratory Specimens

Diagn Microbiol Infect Dis. 2016 Mar;84(3):212-4. doi: 10.1016/j.diagmicrobio.2015.11.001. Epub 2015 Nov 5.


The performance of the Abbott Real Time MTB assay for detection of Mycobacterium tuberculosis complex in respiratory specimens was evaluated using a standard culture as the reference. The overall concordance between both methods was 0.95. The assay displayed an excellent sensitivity (100% for smear-positive/92.3% for smear-negative specimens) and specificity (100%).

Keywords: Acid-fast smear; Culture; Mycobacterium tuberculosis complex; Real-time PCR assays; Respiratory specimens.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / genetics*
  • Reagent Kits, Diagnostic* / standards
  • Real-Time Polymerase Chain Reaction* / methods
  • Real-Time Polymerase Chain Reaction* / standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / microbiology*
  • Young Adult


  • Reagent Kits, Diagnostic