Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-myb

Cell. 1989 Jul 14;58(1):85-93. doi: 10.1016/0092-8674(89)90405-4.

Abstract

The v-myb oncogene, like its cellular progenitor c-myb, encodes a short-lived nuclear protein involved in processes affecting growth and differentiation in a number of cell types. Fusion proteins, in which v-myb sequences are linked to the DNA binding domain of the yeast transcriptional activator GAL4, can activate transcription from a reporter gene linked in cis to a GAL4 binding site. The domain of v-myb responsible for transcriptional activation is located between residues 204 and 254, and is both necessary and sufficient for activation. Intact v-myb and c-myb proteins can also activate transcription, via a myb binding site linked in cis to a reporter gene. A v-myb protein bearing a deletion in the activator domain is no longer capable of stimulating transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Oncogene Proteins, Viral / genetics*
  • Oncogenes
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogenes
  • Recombinant Fusion Proteins / physiology
  • Regulatory Sequences, Nucleic Acid*
  • Transcription Factors / genetics*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • Nuclear Proteins
  • Oncogene Proteins, Viral
  • Proto-Oncogene Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors