Simultaneous detection of single-nucleotide polymorphisms in a DNA bulge structure using a fluorine-modified bisbenzimide derivative

Analyst. 2016 Feb 21;141(4):1214-7. doi: 10.1039/c5an02389k.


Using 3,5-bis(trifluoromethyl)benzene-modified bisbenzimide H33258, bulged-out nucleobases on a DNA strand can be detected by the chemical shift change in (19)F NMR. Based on this technique, single-nucleotide polymorphisms on a DNA sequence can be analyzed simultaneously.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Bisbenzimidazole / chemistry*
  • DNA / chemistry*
  • DNA / genetics*
  • Feasibility Studies
  • Fluorine / chemistry*
  • Models, Molecular
  • Nucleic Acid Conformation*
  • Polymorphism, Single Nucleotide*
  • Time Factors


  • Fluorine
  • DNA
  • Bisbenzimidazole