Powdered coconut water as a storage medium to preserve the viability of periodontal ligament cells: a laboratory study

Int Endod J. 2017 Jan;50(1):84-89. doi: 10.1111/iej.12603. Epub 2016 Jan 19.

Abstract

Aim: To investigate the ability of newly developed powdered coconut water formulas (ACP) with different osmolarities to maintain the viability of periodontal ligament (PDL) cells over time compared with other solutions.

Methodology: Dogs teeth were extracted and stored for two periods, 3 h or 24 h, in the following media: long-shelf life CW (CW), pH-adjusted long-shelf life CW (pH-CW) and powdered CW that was pH and osmolality adjusted (ACP-404-I, 250 mOsm kg-1 H2 O; pH 7.0; ACP-404-II, 372 mOsm kg-1 H2 O; pH 7.0; ACP-404-III, 300 mOsm kg-1 H2 O; pH 7.4). The positive control group (Pc) corresponded to immediate measurement after tooth extraction, and two negative controls (Nc) corresponded to 3 h and 24 h of dry time. PDL cells were extracted, and cell viability analysed by Trypan blue exclusion. Data were analysed statistically using two-way anova followed by the Tukey test and one-way anova followed by the Dunnett test (P < 0.05).

Results: At 3 h and 24 h, ACP-404-I had a performance similar to those of ACP-404-II and pH-CW, with significantly higher (P = 0.004) percentages of viable cells than ACP-404-III and CW. The positive control group had a significantly higher (P = 0.002) percentage of viable cells than the negative control groups, CW and ACP-404-III, irrespective of the period evaluated.

Conclusion: Powdered coconut water formulas, ACP-404-I and ACP-404-II, preserved viability for up to 24 h.

Keywords: cell viability; coconut water; storage media; tooth avulsion.

MeSH terms

  • Animals
  • Cell Survival / drug effects*
  • Cocos*
  • Dogs
  • Organ Preservation Solutions / pharmacology*
  • Osmolar Concentration
  • Periodontal Ligament / cytology*
  • Powders

Substances

  • Organ Preservation Solutions
  • Powders