Synthetic oligonucleotide probes complementary to the HLA-B variable region hybridize with Klebsiella pneumoniae

Microb Pathog. 1989 Apr;6(4):277-86. doi: 10.1016/0882-4010(89)90101-0.


Three oligonucleotide probes complementary to base sequences of the HLA-B variable region were used to probe clinical and foodborne isolates of Klebsiella spp. by DNA colony hybridization. One oligonucleotide (RR-3) corresponding to amino acid residues 66-74 of the HLA-B27.1 sequence and one corresponding to residues 66-74 of the HLA-B7 sequence (RR-5) hybridized with K. pneumoniae under conditions of high stringency. A genomic library was constructed using K. pneumoniae K43 chromosomal DNA, and a 1 kb PstI restriction fragment was found to contain the sequence associated with specific binding of the oligonucleotide probes. After purification and radiolabeling, the cloned fragment hybridized with 96.2% of the K. pneumoniae isolates by DNA colony hybridization. These results confirm the presence of sequence similarities between bacterial DNA and the MHC Class I variable region.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Bacterial / genetics*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • HLA-B Antigens / genetics*
  • Humans
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / immunology
  • Oligonucleotide Probes / chemical synthesis
  • Oligonucleotide Probes / genetics*
  • Plasmids
  • Sequence Homology, Nucleic Acid
  • Spondylitis, Ankylosing / microbiology


  • Antigens, Bacterial
  • DNA, Bacterial
  • HLA-B Antigens
  • Oligonucleotide Probes