Automated data collection in single particle electron microscopy

Microscopy (Oxf). 2016 Feb;65(1):43-56. doi: 10.1093/jmicro/dfv369. Epub 2015 Dec 15.


Automated data collection is an integral part of modern workflows in single particle electron microscopy (EM) research. This review surveys the software packages available for automated single particle EM data collection. The degree of automation at each stage of data collection is evaluated, and the capabilities of the software packages are described. Finally, future trends in automation are discussed.

Keywords: automation; data collection; high throughput; single particle electron microscopy; software packages; target acquisition.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Automation
  • Cryoelectron Microscopy / instrumentation
  • Cryoelectron Microscopy / methods*
  • Data Collection
  • Image Processing, Computer-Assisted / methods*
  • Software