Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field

Sci Rep. 2015 Dec 17;5:18393. doi: 10.1038/srep18393.


The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to noxious stimuli. Thus, this study presents a novel tooth regenerative technology based on split tooth germs and the re-regionalisation of the tooth-forming field by artificial mechanical force.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomechanical Phenomena / genetics
  • Biomechanical Phenomena / physiology
  • Dental Restoration, Permanent / methods*
  • Dental Restoration, Temporary / methods*
  • Gene Expression / genetics
  • In Situ Hybridization
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Odontogenesis / genetics
  • Odontogenesis / physiology*
  • Periodontal Ligament / metabolism
  • Periodontal Ligament / physiology
  • Periodontal Ligament / surgery
  • Regeneration / genetics
  • Regeneration / physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tooth / metabolism
  • Tooth / physiology*
  • Tooth / surgery
  • Tooth Germ / metabolism
  • Tooth Germ / physiology*
  • Tooth Germ / surgery