Brevundimonas albigilva Sp. Nov., Isolated From Forest Soil

Int J Syst Evol Microbiol. 2016 Mar;66(3):1144-1150. doi: 10.1099/ijsem.0.000848. Epub 2015 Dec 16.

Abstract

Strain NHI-13T, a Gram-stain-negative, aerobic and short rod-shaped bacterium, was isolated from forest soil at Kyonggi University in Suwon, South Korea. It grew optimally in R2A medium, at 20-30 °C, in the presence of 0-4 % NaCl. Colonies resulting from incubation of the strain on agar plates for 2 days were circular, raised, translucent, viscous and whitish-yellow, with entire margins. This strain exhibited high catalase activity but was negative for oxidase. 16S rRNA gene sequence analysis showed that strain NHI-13T formed a coherent cluster with members of the genus Brevundimonas. Its similarities were 98.0 % with Brevundimonas aurantiaca DSM 4731T, 97.9 % with Brevundimonas vesicularis LMG 2350T, 97.6 % with Brevundimonas intermedia ATCC 15262T, 97.5 % with Brevundimonas nasdae GTC 1043T, 97.1 % with 'Brevundimonas olei' MJ15, 97.1 % with Brevundimonas mediterranea V4.BO.10T and 97.0 % with Brevundimonas poindexterae FWC40T. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0 and 11-methyl C18 : 1ω7c. The DNA G+C content was 63 mol%. The predominant quinone was ubiquinone Q-10. The polar lipid profile contained 1,2-di-O-acyl-3-O-α-d-glycopyranuronosyl glycerol, 1,2-di-O-acyl-3-O-α-d-glycopyranosyl glycerol, 1,2-di-O-acyl-3-O-[d-glycopyranosyl (1 → 4)-α-d-glucopyranuronosyl] glycerols, phosphatidylglycerol, 1,2-diacyl-3- O-(6'-phosphatidyl-α-d-glucopyranosyl) glycerol and other unknown lipids. The DNA relatedness of strain NHI-13T with its reference strains was in the range of 43-56 %. On the basis of its phenotypic, genotypic, chemotaxonomic and phylogenetic distinctiveness, strain NHI-13T is suggested to be a representative of a novel species, belonging to the genus Brevundimonas. Therefore, the name Brevundimonas albigilva. sp. nov. is proposed, with the type strain being NHI-13T ( = KEME 9005-016T = KACC 18249T = JCM 30385T).