Extensive regulation of nicotinate phosphoribosyltransferase (NAPRT) expression in human tissues and tumors

Oncotarget. 2016 Jan 12;7(2):1973-83. doi: 10.18632/oncotarget.6538.


Nicotinamide adenine dinucleotide (NAD) is a cofactor in redox reactions and a substrate for NAD-consuming enzymes, such as PARPs and sirtuins. As cancer cells have increased NAD requirements, the main NAD salvage enzymes in humans, nicotinamide phosphoribosyltransferase (NAMPT) and nicotinate phosphoribosyltransferase (NAPRT), are involved in the development of novel anti-cancer therapies. Knowledge of the expression patterns of both genes in tissues and tumors is critical for the use of nicotinic acid (NA) as cytoprotective in therapies using NAMPT inhibitors. Herein, we provide a comprehensive study of NAPRT and NAMPT expression across human tissues and tumor cell lines. We show that both genes are widely expressed under normal conditions and describe the occurrence of novel NAPRT transcripts. Also, we explore some of the NAPRT gene expression mechanisms. Our findings underline that the efficiency of NA in treatments with NAMPT inhibitors is dependent on the knowledge of the expression profiles and regulation of both NAMPT and NAPRT.

Keywords: NAMPT; NAPRT; alternative transcripts; anti-cancer therapies; human NAD salvage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Cytokines / genetics*
  • DNA Methylation*
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mutation / genetics*
  • Neoplasms / genetics*
  • Neoplasms / pathology
  • Nicotinamide Phosphoribosyltransferase / genetics*
  • Pentosyltransferases / genetics*
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • Cytokines
  • RNA, Messenger
  • Pentosyltransferases
  • Nicotinamide Phosphoribosyltransferase
  • nicotinamide phosphoribosyltransferase, human
  • nicotinate phosphoribosyltransferase