Synthesis of 1-Naphthol by a Natural Peroxygenase Engineered by Directed Evolution

Chembiochem. 2016 Feb 15;17(4):341-9. doi: 10.1002/cbic.201500493. Epub 2016 Jan 21.

Abstract

There is an increasing interest in enzymes that catalyze the hydroxylation of naphthalene under mild conditions and with minimal requirements. To address this challenge, an extracellular fungal aromatic peroxygenase with mono(per)oxygenase activity was engineered to convert naphthalene selectively into 1-naphthol. Mutant libraries constructed by random mutagenesis and DNA recombination were screened for peroxygenase activity on naphthalene together with quenching of the undesired peroxidative activity on 1-naphthol (one-electron oxidation). The resulting double mutant (G241D-R257K) obtained from this process was characterized biochemically and computationally. The conformational changes produced by directed evolution improved the substrate's catalytic position. Powered exclusively by catalytic concentrations of H2 O2 , this soluble and stable biocatalyst has a total turnover number of 50 000, with high regioselectivity (97 %) and reduced peroxidative activity.

Keywords: 1-naphthol; directed evolution; enzyme catalysis; peroxidative activity; peroxygenases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrocybe / enzymology*
  • Agrocybe / genetics
  • Agrocybe / metabolism
  • Directed Molecular Evolution*
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism*
  • Models, Molecular
  • Naphthalenes / metabolism*
  • Naphthols / metabolism*
  • Point Mutation
  • Protein Engineering*

Substances

  • Naphthalenes
  • Naphthols
  • naphthalene
  • 1-naphthol
  • Mixed Function Oxygenases
  • peroxygenase