Effects of wound dressings on cultured primary keratinocytes

Burns. 2016 Feb;42(1):81-90. doi: 10.1016/j.burns.2015.06.016. Epub 2015 Dec 9.


Autologous cell-spray grafting of non-cultured epidermal cells is an innovative approach for the treatment of severe second-degree burns. After treatment, wounds are covered with dressings that are widely used in wound care management; however, little is known about the effects of wound dressings on individually isolated cells. The sprayed cells have to actively attach, spread, proliferate, and migrate in the wound for successful re-epithelialization, during the healing process. It is expected that exposure to wound dressing material might interfere with cell survival, attachment, and expansion. Two experiments were performed to determine whether some dressing materials have a negative impact during the early phases of wound healing. In one experiment, freshly isolated cells were seeded and cultured for one week in combination with eight different wound dressings used during burn care. Cells, which were seeded and cultured with samples of Adaptic(®), Xeroform(®), EZ Derm(®), and Mepilex(®) did not attach, nor did they survive during the first week. Mepitel(®), N-Terface(®), Polyskin(®), and Biobrane(®) dressing samples had no negative effect on cell attachment and cell growth when compared to the controls. In a second experiment, the same dressings were exposed to pre-cultured cells in order to exclude the effects of attachment and spreading. The results confirm the above findings. This study could be of interest for establishing skin cell grafting therapies in burn medicine and also for wound care in general.

Keywords: Cell transplantation; Dressing toxicity; Epidermal progenitors; Keratinocyte grafting; Keratinocytes; Wound dressing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / genetics
  • Apoptosis / genetics
  • Burns / therapy*
  • Caspase 3 / genetics
  • Cell Culture Techniques
  • Cell Survival
  • Cell Transplantation
  • Cells, Cultured
  • Coated Materials, Biocompatible
  • Gene Expression
  • Humans
  • In Vitro Techniques
  • Integrin alpha6 / genetics
  • Keratinocytes / cytology
  • Keratinocytes / metabolism
  • Keratinocytes / transplantation*
  • Keratins / genetics
  • Ki-67 Antigen / genetics
  • Membrane Proteins / genetics
  • Microscopy, Phase-Contrast
  • Occlusive Dressings*
  • Phenols
  • RNA, Messenger / metabolism*
  • Re-Epithelialization
  • Reverse Transcriptase Polymerase Chain Reaction
  • Silicones
  • Wound Healing
  • fas Receptor / genetics


  • Antigens, CD34
  • Biobrane
  • Coated Materials, Biocompatible
  • Integrin alpha6
  • Ki-67 Antigen
  • Membrane Proteins
  • Phenols
  • RNA, Messenger
  • Silicones
  • fas Receptor
  • loricrin
  • mepitel
  • Keratins
  • CASP3 protein, human
  • Caspase 3
  • 2,4,6-tribromophenol