Double-strand breaks stimulate alternative mechanisms of recombination repair

J Mol Biol. 1989 Jun 5;207(3):527-41. doi: 10.1016/0022-2836(89)90462-2.

Abstract

To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • DNA / genetics*
  • DNA Repair*
  • DNA, Fungal / genetics*
  • Gene Conversion
  • Genes, Fungal
  • Kinetics
  • Models, Genetic
  • Multigene Family
  • Recombination, Genetic
  • Saccharomyces cerevisiae

Substances

  • DNA, Fungal
  • DNA