Flow injection amperometric detection of insulin at cobalt hydroxide nanoparticles modified carbon ceramic electrode

Anal Biochem. 2016 Feb 15:495:37-41. doi: 10.1016/j.ab.2015.11.014. Epub 2015 Dec 11.

Abstract

Cobalt hydroxide nanoparticles were prepared onto a carbon ceramic electrode (CHN|CCE) using the cyclic voltammetry (CV) technique. The modified electrode was characterized by X-ray diffraction and scanning electron microscopy. The results showed that CHN with a single-layer structure was uniformly electrodeposited on the surface of CCE. The electrocatalytic activity of the modified electrode toward the oxidation of insulin was studied by CV. CHN|CCE was also used in a homemade flow injection analysis system for insulin determination. The limit of detection (signal/noise [S/N] = 3) and sensitivity were found to be 0.11 nM and 11.8 nA/nM, respectively. Moreover, the sensor was used for detection of insulin in human serum samples. This sensor showed attractive properties such as high stability, reproducibility, and high selectivity.

Keywords: Carbon ceramic; Cobalt hydroxide; Electrocatalysis; Flow injection analysis; Insulin sensor.

MeSH terms

  • Blood Chemical Analysis / instrumentation*
  • Blood Chemical Analysis / methods*
  • Carbon / chemistry
  • Catalysis
  • Ceramics / chemistry
  • Cobalt / chemistry*
  • Electrochemical Techniques*
  • Electrodes
  • Flow Injection Analysis*
  • Humans
  • Hydroxides / chemistry*
  • Insulin / blood*
  • Insulin / chemistry
  • Limit of Detection
  • Metal Nanoparticles / chemistry*
  • Oxidation-Reduction

Substances

  • Hydroxides
  • Insulin
  • cobalt hydroxide
  • Cobalt
  • Carbon