Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase

Cell Rep. 2015 Dec 22;13(11):2336-2344. doi: 10.1016/j.celrep.2015.11.041. Epub 2015 Dec 10.


Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin's DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Anaphase
  • Centromere / metabolism*
  • Chromosome Segregation
  • Chromosomes, Fungal / metabolism*
  • DNA / chemistry
  • DNA / metabolism
  • DNA Topoisomerases, Type II / metabolism
  • DNA-Binding Proteins / metabolism*
  • Multiprotein Complexes / metabolism*
  • Protein Binding
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / metabolism


  • DNA-Binding Proteins
  • Multiprotein Complexes
  • Saccharomyces cerevisiae Proteins
  • condensin complexes
  • DNA
  • Adenosine Triphosphatases
  • DNA Topoisomerases, Type II