Caryophyllene sesquiterpenoids from the endophytic fungus, Pestalotiopsis sp

Fitoterapia. 2016 Mar:109:119-24. doi: 10.1016/j.fitote.2015.12.003. Epub 2015 Dec 11.

Abstract

Eight new caryophyllene sesquiterpenoids named pestaloporinates A-G (1-7) and 14-acetylhumulane (8) have been isolated from the solid cultures of an endophytic fungus Pestalotiopsis sp., which was obtained from the fresh stem bark of Melia azedarach Linn. Their structures as well as absolute configurations were determined by spectroscopic data, ECD experimentation, and single-crystal X-ray diffraction. Among all the isolates, compound 2 displayed potent inhibitory activity with IC50 value of 19.0 μM during the evaluation of nitric oxide (NO) inhibition in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells.

Keywords: Caryophyllene sesquiterpenoids; Ethyl ethanoate (PubChem CID: 8857); Methanol (PubChem CID: 887); Methyl cyanide (PubChem CID: 6342); Methylene chloride (PubChem CID: 6344); NO inhibition; Pestalotiopsis sp.; Pyridine (PubChem CID: 1049).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification
  • Macrophages / drug effects
  • Melia azedarach / microbiology
  • Mice
  • Molecular Structure
  • Nitric Oxide / metabolism
  • Plant Bark / microbiology
  • Polycyclic Sesquiterpenes
  • RAW 264.7 Cells
  • Sesquiterpenes / chemistry*
  • Sesquiterpenes / isolation & purification
  • Xylariales / chemistry*

Substances

  • Anti-Inflammatory Agents
  • Polycyclic Sesquiterpenes
  • Sesquiterpenes
  • pestaloporinate B
  • Nitric Oxide
  • caryophyllene