Stray light correction in the optical spectroscopy of crystals

Appl Spectrosc. 2015 Sep;69(9):1106-11. doi: 10.1366/14-07716.


It has long been known in spectroscopy that light not passing through a sample, but reaching the detector (i.e., stray light), results in a distortion of the spectrum known as absorption flattening. In spectroscopy with crystals, one must either include such stray light or take steps to exclude it. In the former case, the derived spectra are not accurate. In the latter case, a significant amount of the crystal must be masked off and excluded. In this paper, we describe a method that allows use of the entire crystal by correcting the distorted spectrum.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Crystallography / methods*
  • Kinetics
  • Light
  • Purple Membrane / chemistry*
  • Scattering, Radiation*
  • Spectrum Analysis / methods*