Reproducible E. coli detection based on label-free SERS and mapping

Danting Yang; Haibo Zhou; Christoph Haisch; Reinhard Niessner; Yibin Ying

doi:10.1016/j.talanta.2015.09.006

Reproducible E. coli detection based on label-free SERS and mapping

Talanta. 2016:146:457-63. doi: 10.1016/j.talanta.2015.09.006. Epub 2015 Sep 8.

Authors

Danting Yang¹, Haibo Zhou², Christoph Haisch³, Reinhard Niessner³, Yibin Ying⁴

Affiliations

¹ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
² Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Munich, Germany; Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, China.
³ Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Munich, Germany.
⁴ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China. Electronic address: ibeying@zju.edu.cn.

PMID: 26695290
DOI: 10.1016/j.talanta.2015.09.006

Abstract

The biosensing for rapid detection of bacteria based on surface-enhanced Raman scattering (SERS) has been widely explored for recent years. It is still a challenge to achieve a high sensitive, reproducible label free detection method for bacteria. In this work, a label-free SERS detection method of Escherichia coli based on incubation with silver colloid was reported. Optimized incubation conditions including shaking speed, time and temperature were used to help construct a rapid SERS method for E. coli analysis. It was found that the enhancement of the Raman signal of E. coli could be achieved to 1.8×10(4) cps (counts per second) with high reproducibility. Three strains of E. coli DSM 1116/498/5695 could be successfully discriminated using such SERS method combining discriminant analysis. Finally, the lowest concentration of E. coli at 1×10(5) cell/mL can be detected by SERS mapping. Thus, our detection method offers higher sensitivity and reproducibility compared to previously reported label free simple-mixing methods, opening an avenue for developing various SERS-based biosensor.

Keywords: Discriminant analysis; E. coli; Label-free; SERS; Single bacterium cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / chemistry
Escherichia coli / isolation & purification*
Limit of Detection
Reproducibility of Results
Spectrum Analysis, Raman / instrumentation
Spectrum Analysis, Raman / methods*
Temperature