Topical application of Aloe vera and vitamin E on induced ulcers on the tongue of rats subjected to radiation: clinical and histological evaluation

Support Care Cancer. 2016 Jun;24(6):2557-64. doi: 10.1007/s00520-015-3048-3. Epub 2015 Dec 23.


Objective: The aim of this study is to assess the effect of two types of antioxidants, vitamin E (VE) and Aloe vera (AV), on healing of induced oral lesions after radiation in a murine model by clinical and histological analysis.

Methods: The animals were randomly divided into three groups of 12 animals each (400 mg VE, 70 % AV and control) and two time periods (5 and 7 days). They were irradiated with a single dose of 30 Gy, and after 24 h, a lesion was produced on the ventral tongue of each animal. The products were applied daily in their respective group until euthanasia.

Results: On clinical analysis, there was a higher frequency of lesions in the animals of the control group at both periods. The area of the lesions was also greater in the control group compared with the groups AV and VE (5 days p = 0.006; 7 days p = 0.002). On microscopic analysis, the degree of inflammation differed between the study groups and experimental periods. At 5 days, the statistical difference was not significant among the groups evaluated, but at 7 days, animals in the control group showed intense inflammation, while those in groups VE and AV exhibited mild to moderate inflammation (p = 0.002).

Conclusion: The results suggest that VE and AV contributed to the decrease in inflammatory response and healing of the lesions induced on the tongue of rats subjected to radiation.

Keywords: Antioxidants; Oral medicine; Oral mucositis; Radiotherapy.

MeSH terms

  • Administration, Topical
  • Aloe*
  • Animals
  • Antioxidants / administration & dosage
  • Disease Models, Animal
  • Female
  • Male
  • Oral Ulcer / drug therapy*
  • Oral Ulcer / etiology
  • Plant Extracts / administration & dosage*
  • Radiation Injuries, Experimental / drug therapy*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Tongue Diseases / drug therapy*
  • Tongue Diseases / etiology
  • Vitamin E / administration & dosage*
  • Wound Healing / drug effects


  • Antioxidants
  • Plant Extracts
  • Vitamin E