Abstract
Bioactivity-guided isolation of a methanolic extract of Euphorbia fischeriana led to the isolation of four new abietane-type diterpenoids, fischeriolides A-D (1-4), together with 11 known diterpenoids. Their structures were elucidated based on the interpretation of 1D and 2D NMR spectroscopic and HRESIMS data. The absolute configuration of compound 3 was determined by single-crystal X-ray diffraction analysis and electronic circular dichroism methods. Compounds 5-9 exhibited inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages with IC50 values in the range 4.9-12.6 μM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry
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Crystallography, X-Ray
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology*
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology*
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Euphorbia / chemistry*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Mice
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Molecular Conformation
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Molecular Structure
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Nitric Oxide / biosynthesis*
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Plant Roots / chemistry
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Republic of Korea
Substances
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Antineoplastic Agents, Phytogenic
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Diterpenes
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Drugs, Chinese Herbal
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Lipopolysaccharides
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Nitric Oxide