Influence of therapeutic radiation on polycaprolactone and polyurethane biomaterials

Shelley L Cooke; Abby R Whittington

doi:10.1016/j.msec.2015.10.089

Influence of therapeutic radiation on polycaprolactone and polyurethane biomaterials

Mater Sci Eng C Mater Biol Appl. 2016 Mar:60:78-83. doi: 10.1016/j.msec.2015.10.089. Epub 2015 Oct 31.

Authors

Shelley L Cooke¹, Abby R Whittington²

Affiliations

¹ Department of Materials Science and Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
² Department of Materials Science and Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. Electronic address: awhit@mse.vt.edu.

PMID: 26706509
DOI: 10.1016/j.msec.2015.10.089

Abstract

Biomedical polymers are exposed in vivo to ionizing radiation as implants, coatings and bystander materials. High levels of ionizing radiation (e.g. X-ray and gamma) have been reported to cause degradation and/or cross-linking in many polymers. This pilot study sought to determine causes of failure, by investigating how therapeutic radiation affects two different porous polymeric scaffolds: polycaprolactone (PCL) and polyurethane (PU). PCL is a bioresorbable material used in biomedical devices (e.g., dentistry, internal fixation devices and targeted drug delivery capsules). PU is commonly used in medical applications (e.g., coatings for pacemakers, tissue expanders, catheter tubing and wound dressings). PU was specifically fabricated to be a non-degradable polymer in this study. Porous scaffolds, fabricated using solvent casting and/or salt leeching techniques, were placed in phosphate buffered saline (PBS, pH=7.4) and exposed to typical cancer radiotherapy. A total dose of 50 Gy was broken into 25 doses over an eleven-week period. Collected PBS was tested for polymer leachants and degradation products using Gas Chromatography Mass Spectroscopy (GC-MS), results revealed no analyzable leachants from either polymer. Scaffolds were characterized using Environmental Scanning Electron Microscopy, Size-exclusion chromatography (SEC), Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared Spectroscopy (FTIR). No gross visual changes were observed in either polymer, however PU exhibited microstructure changes after irradiation. Increased number average molecular weight and weight average molecular weight in PCL and PU were observed after irradiation, indicating crosslinking. PU displayed an increase in intrinsic viscosity that further confirms increased crosslinking. PCL and PU showed decreases in crystallinity after irradiation, and PU crystallinity shifted from long-range-order hard segments to short-range-order hard segments after irradiation. Results from both PCL and PU suggest changes in polymer backbones. This preliminary study suggests that therapeutic radiation doses cause both degradation and crosslinking in PCL and PU.

Keywords: Polycaprolactone; Polyurethane; Porous scaffolds; Therapeutic radiation.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biocompatible Materials / chemistry*
Polyesters / chemistry*
Polyurethanes / chemistry*
Tissue Scaffolds / chemistry*

Substances

Biocompatible Materials
Polyesters
Polyurethanes
polycaprolactone