Strategies for production of butanol and butyl-butyrate through lipase-catalyzed esterification

Bioresour Technol. 2016 Feb:202:214-9. doi: 10.1016/j.biortech.2015.11.068. Epub 2015 Dec 10.

Abstract

In this study, a fermentation process for production of butanol and butyl-butyrate by using Clostridium sp. strain BOH3 is developed. This strain is able to produce butyric acid and butanol when it ferments 60 g/L xylose. Meanwhile, it also excreted indigenous lipases (induced by olive oil) which naturally convert butyric acid and butanol into 1.2 g/L of butyl-butyrate. When Bio-OSR was used as both an inducer for lipase and extractant for butyl-butyrate, the butyl-butyrate concentration can reach 6.3 g/L. To further increase the yield, additional lipases and butyric acid are added to the fermentation system. Moreover, kerosene was used as an extractant to remove butyl-butyrate in situ. When all strategies are combined, 22.4 g/L butyl-butyrate can be produced in a fed-batch reactor spiked with 70 g/L xylose and 7.9 g/L butyric acid, which is 4.5-fold of that in a similar system (5 g/L) with hexadecane as the extractant.

Keywords: Butanol; Butyl-butyrate; Clostridium; Extractant; Lipase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Butanol / metabolism
  • Bioreactors
  • Butanols / metabolism*
  • Butyrates / metabolism*
  • Butyric Acid / metabolism
  • Catalysis
  • Clostridium / metabolism
  • Esterification
  • Fermentation*
  • Lipase / metabolism*
  • Xylose / metabolism

Substances

  • Butanols
  • Butyrates
  • Butyric Acid
  • n-butyl n-butyrate
  • 1-Butanol
  • Xylose
  • Lipase