Accelerated Evolution of Enhancer Hotspots in the Mammal Ancestor

Mol Biol Evol. 2016 Apr;33(4):1008-18. doi: 10.1093/molbev/msv344. Epub 2015 Dec 29.


Mammals have evolved remarkably different sensory, reproductive, metabolic, and skeletal systems. To explore the genetic basis for these differences, we developed a comparative genomics approach to scan whole-genome multiple sequence alignments to identify regions that evolved rapidly in an ancestral lineage but are conserved within extant species. This pattern suggests that ancestral changes in function were maintained in descendants. After applying this test to therian mammals, we identified 4,797 accelerated regions, many of which are noncoding and located near developmental transcription factors. We then used mouse transgenic reporter assays to test if noncoding accelerated regions are enhancers and to determine how therian-specific substitutions affect their activity in vivo. We discovered enhancers with expression specific to the therian version in brain regions involved in the hormonal control of milk ejection, uterine contractions, blood pressure, temperature, and visual processing. This work underscores the idea that changes in developmental gene expression are important for mammalian evolution, and it pinpoints candidate genes for unique aspects of mammalian biology.

Keywords: comparative genomics; development; enhancer evolution; mammal-specific traits.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Conserved Sequence / genetics
  • Enhancer Elements, Genetic*
  • Evolution, Molecular*
  • Gene Expression Regulation, Developmental
  • Genomics
  • Homeodomain Proteins / genetics*
  • Mammals / genetics*
  • Mice


  • Homeodomain Proteins