Genetic requirements for hyper-recombination by very short patch mismatch repair: involvement of Escherichia coli DNA polymerase I

Mol Gen Genet. 1989 Jun;217(2-3):254-6. doi: 10.1007/BF02464889.


It has been established that very short patch (VSP) mismatch repair, depending in Escherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme. The absence of endonuclease activities involved in the repair of base-loss sites, Nth, Nfo and Xth, does not affect VSP repair. Implications for the mechanism of the VSP repair are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Base Sequence
  • Crosses, Genetic
  • DNA Polymerase I / metabolism*
  • DNA Repair*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Genetic Markers
  • Mutation
  • Recombination, Genetic


  • Genetic Markers
  • DNA Polymerase I