Nucleotide Sequence of a cDNA Coding for the NADPH-protochlorophyllide Oxidoreductase (PCR) of Barley (Hordeum Vulgare L.) and Its Expression in Escherichia Coli

Mol Gen Genet. 1989 Jun;217(2-3):355-61. doi: 10.1007/BF02464904.

Abstract

The primary structure of the NADPH-protochlorophyllide oxidoreductase of barley has been deduced from the nucleotide sequence of a cloned full-length cDNA. This cDNA hybridizes to a 1.7 kb RNA whose steady-state level in dark-grown seedlings is drastically reduced upon illumination. The predicted amino acid sequence (388 residues in length) includes a transit peptide of 74 amino acids whose end point has been delimited by sequencing the N-terminus of the mature protein. Expression of the cDNA in Escherichia coli leads to the synthesis of an enzymatically active precursor of the NADPH-protochlorophyllide oxidoreductase. Activity of this protein in bacterial lysates is completely dependent on the presence of NADPH and protochlorophyllide and requires light.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Escherichia coli / genetics
  • Molecular Sequence Data
  • Oxidoreductases / genetics*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Plants / enzymology
  • Plants / genetics*

Substances

  • DNA
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • protochlorophyllide reductase

Associated data

  • GENBANK/X15869