A novel Mn(2+)-doped core/shell quantum dot-based intracellular probe for fluoride anions sensing in MC3T3-E1 osteoblastic cells

Talanta. 2016:149:285-289. doi: 10.1016/j.talanta.2015.12.001. Epub 2015 Dec 2.

Abstract

In this paper, 3-aminobenzeneboronic acid functionalized Mn(2+)-doped ZnTe/ZnSe quantum dots (APBA-dQDs) were prepared. The APBA functional groups had strong binding ability with F(-), resulting in the quenchment of dQDs photoluminescence (PL). Under the optimal condition, the fluorescence intensity of APBA-dQDs was related linearly to the concentration of F(-) in the range of 0.25-1.5µmol/L with a detection limit of 0.1µmol/L. The selectivity of fluorescence quenching of APBA-dQDs for F(-) was enhanced. Moreover, the proposed methodology for the sensing of F(-) at EM 560nm in MC3T3-E1 osteoblastic cells was demonstrated and got a satisfactory results. The results indicate that the APBA-dQDs are promising candidates for intracellular in MC3T3-E1 osteoblastic cells. To the best of our knowledge, it was the first report of F(-) sensing by using the quenched fluorescence of APBA-dQDs in non-cancerous cells.

Keywords: F(–) sensing; Intracellular sensor; MC3T3-E1 osteoblastic cells; Quantum dots.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Boronic Acids / chemistry*
  • Cell Line
  • Fluorides / analysis*
  • Fluorides / chemistry
  • Manganese / chemistry*
  • Mice
  • Osteoblasts
  • Quantum Dots / chemistry*
  • Selenium Compounds / chemistry*
  • Tellurium / chemistry*
  • Zinc Compounds / chemistry*

Substances

  • Boronic Acids
  • Selenium Compounds
  • Zinc Compounds
  • 3-aminobenzeneboronic acid
  • Manganese
  • Tellurium
  • zinc selenide
  • Fluorides