Zebrafish Plzf transcription factors enhance early type I IFN response induced by two non-enveloped RNA viruses

Dev Comp Immunol. 2016 Apr;57:48-56. doi: 10.1016/j.dci.2015.12.016. Epub 2015 Dec 21.


The BTB-POZ transcription factor Promyelocytic Leukemia Zinc Finger (PLZF, or ZBTB16) has been recently identified as a major factor regulating the induction of a subset of Interferon stimulated genes in human and mouse. We show that the two co-orthologues of PLZF found in zebrafish show distinct expression patterns, especially in larvae. Although zbtb16a/plzfa and zbtb16b/plzfb are not modulated by IFN produced during viral infection, their over-expression increases the level of the early type I IFN response, at a critical phase in the race between the virus and the host response. The effect of Plzfb on IFN induction was also detectable after cell infection by different non-enveloped RNA viruses, but not after infection by the rhabdovirus SVCV. Our findings indicate that plzf implication in the regulation of type I IFN responses is conserved across vertebrates, but at multiple levels of the pathway and through different mechanisms.

Keywords: Antiviral innate immunity; Interferon; PLZF; ZBTB; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Immunity, Innate
  • Interferon Type I / immunology*
  • Interferon Type I / metabolism
  • Kruppel-Like Transcription Factors / classification
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism*
  • Mice
  • Phylogeny
  • Poly I-C / immunology
  • Promyelocytic Leukemia Zinc Finger Protein
  • RNA Virus Infections / immunology*
  • RNA Viruses / immunology*
  • RNA, Viral / immunology
  • Transcriptome
  • Zebrafish / genetics
  • Zebrafish / immunology*
  • Zebrafish Proteins / classification
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism*


  • Interferon Type I
  • Kruppel-Like Transcription Factors
  • Promyelocytic Leukemia Zinc Finger Protein
  • RNA, Viral
  • Zbtb16 protein, mouse
  • Zebrafish Proteins
  • zbtb16a protein, zebrafish
  • ZBTB16 protein, human
  • Poly I-C