Hemagglutinin Stalk Immunity Reduces Influenza Virus Replication and Transmission in Ferrets

Raffael Nachbagauer; Matthew S Miller; Rong Hai; Alex B Ryder; John K Rose; Peter Palese; Adolfo García-Sastre; Florian Krammer; Randy A Albrecht

doi:10.1128/JVI.02481-15

Hemagglutinin Stalk Immunity Reduces Influenza Virus Replication and Transmission in Ferrets

J Virol. 2015 Dec 30;90(6):3268-73. doi: 10.1128/JVI.02481-15.

Authors

Raffael Nachbagauer¹, Matthew S Miller², Rong Hai³, Alex B Ryder⁴, John K Rose⁴, Peter Palese⁵, Adolfo García-Sastre⁶, Florian Krammer⁷, Randy A Albrecht⁸

Affiliations

¹ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Faculty of Life Sciences, University of Vienna, Vienna, Austria.
² Department of Biochemistry and Biomedical Sciences, Institute for Infectious Diseases Research, McMaster Immunology Research Centre, McMaster University, Hamilton, Ontario, Canada.
³ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
⁴ Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, USA.
⁵ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
⁶ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA Global Health & Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
⁷ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA randy.albrecht@mssm.edu florian.krammer@mssm.edu.
⁸ Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Global Health & Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA randy.albrecht@mssm.edu florian.krammer@mssm.edu.

Abstract

We assessed whether influenza virus hemagglutinin stalk-based immunity protects ferrets against aerosol-transmitted H1N1 influenza virus infection. Immunization of ferrets by a universal influenza virus vaccine strategy based on viral vectors expressing chimeric hemagglutinin constructs induced stalk-specific antibody responses. Stalk-immunized ferrets were cohoused with H1N1-infected ferrets under conditions that permitted virus transmission. Hemagglutinin stalk-immunized ferrets had lower viral titers and delayed or no virus replication at all following natural exposure to influenza virus.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Disease Models, Animal
Disease Transmission, Infectious / prevention & control*
Ferrets
Hemagglutinin Glycoproteins, Influenza Virus / immunology*
Influenza A Virus, H1N1 Subtype / immunology*
Influenza A Virus, H1N1 Subtype / physiology
Influenza Vaccines / administration & dosage
Influenza Vaccines / immunology*
Male
Orthomyxoviridae Infections / prevention & control*
Orthomyxoviridae Infections / transmission*
Orthomyxoviridae Infections / virology
Viral Load
Virus Replication / immunology

Substances

H1N1 virus hemagglutinin
Hemagglutinin Glycoproteins, Influenza Virus
Influenza Vaccines

Abstract

Publication types

MeSH terms

Substances

Grants and funding