Isolation and Characterization of a Novel Bat Coronavirus Closely Related to the Direct Progenitor of Severe Acute Respiratory Syndrome Coronavirus

J Virol. 2015 Dec 30;90(6):3253-6. doi: 10.1128/JVI.02582-15.


We report the isolation and characterization of a novel bat coronavirus which is much closer to the severe acute respiratory syndrome coronavirus (SARS-CoV) in genomic sequence than others previously reported, particularly in its S gene. Cell entry and susceptibility studies indicated that this virus can use ACE2 as a receptor and infect animal and human cell lines. Our results provide further evidence of the bat origin of the SARS-CoV and highlight the likelihood of future bat coronavirus emergence in humans.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin-Converting Enzyme 2
  • Animals
  • Cell Line
  • Chiroptera / virology*
  • Coronavirus / classification*
  • Coronavirus / genetics
  • Coronavirus / isolation & purification*
  • Coronavirus / physiology
  • Humans
  • Molecular Sequence Data
  • Peptidyl-Dipeptidase A / metabolism
  • Phylogeny*
  • RNA, Viral / genetics
  • Receptors, Virus / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology
  • Severe acute respiratory syndrome-related coronavirus / genetics
  • Spike Glycoprotein, Coronavirus / genetics
  • Virus Attachment


  • RNA, Viral
  • Receptors, Virus
  • Spike Glycoprotein, Coronavirus
  • Peptidyl-Dipeptidase A
  • ACE2 protein, human
  • Angiotensin-Converting Enzyme 2