LDH-A promotes malignant progression via activation of epithelial-to-mesenchymal transition and conferring stemness in muscle-invasive bladder cancer

Biochem Biophys Res Commun. 2016 Jan 22;469(4):985-92. doi: 10.1016/j.bbrc.2015.12.078. Epub 2015 Dec 22.

Abstract

Lactate dehydrogenase-A(LDH-A) is an important rate-limiting enzyme in the Warburg effect. Survival analysis indicated poor clinical outcomes in MIBC with high LDH-A expression. The results of in vitro experiment indicated that LDH-A promotes MIBC cells proliferation, invasion and migration. The positive relationship between LDH-A expression and CSC/EMT markers was confirmed both in invasive bladder cell line and in 136 MIBC specimens. Thus, we conclude that LDH-A may be a promising target for MIBC.

Keywords: E-cadherin; Epithelial-to-mesenchymal transition; Histone deacetylases; Lactate dehydrogenase-A; Muscle-invasive bladder cancer; Oct4.

MeSH terms

  • Biomarkers, Tumor / metabolism
  • Carcinogenesis / metabolism*
  • Carcinogenesis / pathology
  • Cell Differentiation
  • Cell Line, Tumor
  • China / epidemiology
  • Epithelial-Mesenchymal Transition*
  • Female
  • Humans
  • Isoenzymes / metabolism
  • L-Lactate Dehydrogenase / metabolism*
  • Lactate Dehydrogenase 5
  • Male
  • Middle Aged
  • Muscle, Smooth / enzymology
  • Muscle, Smooth / pathology
  • Neoplasm Invasiveness
  • Neoplastic Stem Cells / enzymology
  • Neoplastic Stem Cells / pathology*
  • Prevalence
  • Survival Analysis
  • Urinary Bladder Neoplasms / enzymology*
  • Urinary Bladder Neoplasms / mortality*
  • Urinary Bladder Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • Isoenzymes
  • L-Lactate Dehydrogenase
  • Lactate Dehydrogenase 5