Far-red fluorescent cell line for preclinical study of HER2-targeted agents

Dokl Biochem Biophys. 2015;465:410-2. doi: 10.1134/S1607672915060174. Epub 2016 Jan 5.

Abstract

We have created novel HER2-overexpressing human ovarian adenocarcinoma cell line stably transfected with far-red fluorescent protein TurboFP635. Growth characteristics, adhesion capacity and morphology of the cells do not differ from that of parental cell line. The obtained cell line (SKOV-kat) was confirmed to have high level of expression of the tumor marker HER2 comparable to that of initial cell line SKOV-3. The SKOV-kat cell line is designed for further establishment of experimental fluorescent models of HER2-overexpressing human ovarian cancer that make it possible to optimize and reduce the cost of preclinical study of agents for treatment of this disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Cell Culture Techniques / methods*
  • Cell Line
  • Drug Evaluation, Preclinical / methods*
  • Female
  • Humans
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism*
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology*
  • Receptor, ErbB-2 / genetics
  • Receptor, ErbB-2 / metabolism*

Substances

  • Luminescent Proteins
  • red fluorescent protein
  • ERBB2 protein, human
  • Receptor, ErbB-2