Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Dec;77(Suppl 2):551-6.
doi: 10.1007/s12262-013-0919-z. Epub 2013 May 18.

Osteopontin Expression in Patients With Hepatolith

Affiliations
Free PMC article

Osteopontin Expression in Patients With Hepatolith

Bum Soo Kim et al. Indian J Surg. .
Free PMC article

Abstract

To investigate the role of osteopontin (OPN) in the formation of hepatolith, we have immunohistochemically studied the involvement of OPN in hepatolithiasis in the intrahepatic bile ducts and the intramural and extramural glands, and in stones. In our hospital, 17 hepatic resections for hepatolithiasis were carried out from June 2006 to October 2009. Immunohistochemistry was performed on 17 liver specimens with hepatolith and on 24 control liver specimens without hepatolith. We compared the osetopontin expression in the epithelia of the intrahepatic bile ducts and peribiliary glands or periglandular macrophages. Staining for OPN was positive in the cytoplasm of the epithelial cells of stone-containing intrahepatic bile ducts and the intramural and extramural glands, and in stones. But there were no significant differences between the hepatolithiasis group and the control group for the OPN immunoreactivity of the luminal surface of the epithelial cells of the intrahepatic bile ducts and intramural and extramural glands. The stone-containing intrahepatic bile ducts were infiltrated by macrophages that showed intense staining for OPN. The core and matrix of the stones showed OPN immunoreactivity. The degree of OPN from the cytoplasm of the epithelia of the intrahepatic bile ducts and peribiliary glands or periglandular macrophages was different between hepatolithiasis and control groups. Our result suggests that the OPN from the intrahepatic bile ducts and peribiliary glands plays a role in the formation of intrahepatic stone.

Keywords: Hepatolithiasis; Immunohistochemistry; Intrahepatic bile duct; Osteopontin.

Figures

Fig. 1
Fig. 1
Osteopontin immunoreactivity in the control group showed negative immunostaining in the cytoplasm and on the luminal surface of the epithelial cells in the intrahepatic duct (×400)
Fig. 2
Fig. 2
a Positive immunostaining is seen in the cytoplasm and on the luminal surface of the epithelial cells of the intrahepatic duct. Negative immunostaining is seen in the cytoplasm of the intramural glands (×400). b Positive immunostaining is seen in the cytoplasm and on the luminal surface of the epithelial cells of the intrahepatic duct and intramural glands (×400)
Fig. 3
Fig. 3
Cytoplasmic expression of OPN in the extramural gland cells. Negative immunostaining is seen in the cytoplasm and on the luminal surface (×400)
Fig. 4
Fig. 4
Cytoplasmic expression of OPN in the extramural gland cells. Positive immunostaining is seen in the cytoplasm and on the luminal surface (×400)
Fig. 5
Fig. 5
OPN-positive macrophages (arrow) are seen in the epithelial layer of the intrahepatic duct. OPN immunostaining (×400)
Fig. 6
Fig. 6
Immunohistochemical staining of calcium bilirubinate stone. The lamellar pattern is demonstrated by OPN immunostaining (×200)

Similar articles

See all similar articles

Cited by 1 article

LinkOut - more resources

Feedback