Diacylglycerols, unlike phorbol esters, do not induce homologous desensitization or down-regulation of protein kinase C in Swiss 3T3 cells

Biochem Biophys Res Commun. 1989 Aug 30;163(1):201-8. doi: 10.1016/0006-291x(89)92121-9.

Abstract

Addition of 1-oleoyl-2-acetyl-glycerol (OAG), 1,2-dioctanoyl-glycerol (diC8) or phorbol-12, 13-dibutyrate (PDBu) to cultures of Swiss 3T3 cells rapidly increases the phosphorylation of the Mr 80,000 protein kinase C (PKC) substrate, inhibits EGF binding and stimulates DNA synthesis. Prolonged incubation (40 h) with PDBu completely blocked these responses to all agents and down-regulated PKC. In contrast, a similar treatment with OAG or diC8, at mitogenic concentrations, neither induced homologous cellular desensitization nor decreased the immunoreactive level or activity of PKC. The results show that PKC down-regulation can be dissociated from PKC-mediated mitogenesis in Swiss 3T3 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cell Line
  • Diglycerides / pharmacology*
  • Enzyme Activation / drug effects
  • Glycerides / pharmacology*
  • Insulin / pharmacology
  • Mice
  • Phorbol Esters / pharmacology*
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Protein Kinase C / metabolism*

Substances

  • Diglycerides
  • Glycerides
  • Insulin
  • Phorbol Esters
  • Phosphoproteins
  • Protein Kinase C