IL-10 downregulates CXCR3 expression on Th1 cells and interferes with their migration to intestinal inflammatory sites

Mucosal Immunol. 2016 Sep;9(5):1263-77. doi: 10.1038/mi.2015.132. Epub 2016 Jan 6.


Inflammatory bowel disease (IBD) is characterized by chronic, uncontrolled inflammation in the intestinal mucosa. Although the etiology is poorly understood, it is widely accepted that loss of tolerance is involved in the development of IBD. Therefore, re-establishing tolerance or gut homeostasis is one of the key features in the development of new therapeutic strategies. Here we show that antigen targeting to DEC-205 on dendritic cells leads to an interleukin (IL)-10-dependent downregulation of C-X-C chemokine receptor 3 (CXCR3) expression on differentiated antigen-specific T helper type 1 (Th1) cells in vivo. This downregulation interferes with the migration of Th1 cells into the gut and protects mice against severe acute and relapsing intestinal inflammation. Moreover, CD4(+)CXCR3(+) T cells are highly enriched in the inflamed mucosa of IBD patients. Interference with this pathway may therefore be a promising approach for the treatment of IBD. In conclusion, we propose a hitherto undescribed mechanism by which IL-10 can act on effector T cells and orchestrate intestinal immune responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / immunology*
  • CD4 Antigens / genetics
  • CD4 Antigens / immunology
  • Cell Movement
  • Colitis, Ulcerative / genetics
  • Colitis, Ulcerative / immunology*
  • Colitis, Ulcerative / pathology
  • Crohn Disease / genetics
  • Crohn Disease / immunology*
  • Crohn Disease / pathology
  • Dendritic Cells / immunology
  • Dendritic Cells / pathology
  • Gene Expression Regulation
  • Humans
  • Immune Tolerance
  • Interleukin-10 / genetics
  • Interleukin-10 / immunology*
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / pathology
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Minor Histocompatibility Antigens / genetics
  • Minor Histocompatibility Antigens / immunology*
  • Receptors, CXCR3 / genetics
  • Receptors, CXCR3 / immunology*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology*
  • Signal Transduction
  • Th1 Cells / immunology*
  • Th1 Cells / pathology


  • Antigens, CD
  • CD4 Antigens
  • Cxcr3 protein, mouse
  • DEC-205 receptor
  • IL10 protein, mouse
  • Lectins, C-Type
  • Minor Histocompatibility Antigens
  • Receptors, CXCR3
  • Receptors, Cell Surface
  • Interleukin-10