Detection of Legionella pneumophila in environmental water samples using a fluorescein conjugated monoclonal antibody

Epidemiol Infect. 1989 Aug;103(1):105-12. doi: 10.1017/s0950268800030405.


Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. GS detected L. pneumophila in 94% and 100% of environmental water samples which were culture and IFAT positive for L. pneumophila, respectively. IFAT detected 69% of L. pneumophila culture positive samples. Cultures of L. pneumophila serogroups 1 to 12, 14 and non-L. pneumophila bacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. GS identified all serogroups of L. pneumophila and did not cross react with any non-L. pneumophila bacteria. L. pneumophila in environmental samples was easy to detect against a clear dark background when stained with GS.

MeSH terms

  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal*
  • Antigens, Bacterial / analysis
  • Fluoresceins
  • Fluorescent Antibody Technique*
  • Immune Sera
  • Legionella / immunology
  • Legionella / isolation & purification*
  • Predictive Value of Tests
  • Water Microbiology*


  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Fluoresceins
  • Immune Sera