Visualisation of newly synthesised collagen in vitro and in vivo

Sci Rep. 2016 Jan 7:6:18780. doi: 10.1038/srep18780.


Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize newly deposited collagen based on its inherent association with dermatan sulfate. The method is applicable irrespective of host species and collagen source.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials
  • Cells, Cultured
  • Collagen / biosynthesis*
  • Dermatan Sulfate / biosynthesis
  • Dogs
  • Fibroblasts / metabolism
  • Humans
  • Implants, Experimental
  • Mice
  • Rats
  • Sus scrofa
  • Tissue Engineering


  • Biocompatible Materials
  • Dermatan Sulfate
  • Collagen