Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward

Trends Cell Biol. 2016 Feb;26(2):77-79. doi: 10.1016/j.tcb.2015.11.005. Epub 2015 Dec 28.

Abstract

Gene editing in zebrafish has begun to reveal discordance between mutant phenotypes and those associated with knockdown via morpholino oligonucleotides (MOs). These studies suggest that MOs should not be used as a standalone tool and underscore the need for guidelines that require defined mutants to assess gene function in zebrafish.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Gene Knockdown Techniques / methods
  • Gene Knockdown Techniques / trends
  • Humans
  • Morpholinos / genetics
  • Mutagenesis / physiology
  • Mutation / genetics
  • Reverse Genetics* / methods
  • Reverse Genetics* / trends
  • Zebrafish / genetics*

Substances

  • Morpholinos